ABTS HRP MICROWELL SUBSTRATE

ABTS (2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt) is commonly used as a substrate with hydrogen peroxide for a peroxidase enzyme or alone with a laccase enzyme. Its use allows the reaction kinetics of peroxidases themselves to be followed. In this way it also can be used to indirectly follow the reaction kinetics of any hydrogen peroxide producing enzyme, or to simply quantify the amount of hydrogen peroxide in a sample. is a water-soluble HRP substrate that yields a green end product upon reaction with peroxidase. The green product has two major absorbance peaks, 410 nm and 650 nm. ABTS is less sensitive than TMB in ELISA applications, it is less readily oxidized, and the kinetics of its color development is considerably slower.  BioFX Laboratories has created a one component ABTS for microwell applications.  An ABTS enhancer is also available to increase the sensitivity of your assay up to 80%.