AP CHEMILUMINESCENT SUBSTRATES
BioFX Laboratories offers five unique AP chemiluminescent substrates to meet all your   sensitivity requirements for both microwell and membrane applications.  These one component formulations are sensitive in the attogram to nanogram range and are available as reagents for both 450 nm and 540 nm.  BioFX chemiluminescent substrates are characterized by varying lengths of light emission and intensity, allowing you to choose desired sensitivity levels for your assay.  Light is produced only while the enzyme (AP) and substrate reaction is occurring.  This is in contrast to colorimetric substrates, where a stable color is produced and the resulting precipitates remain on the membrane, even after the enzyme-substrate reaction has completed. For Western blotting, chemiluminescent substrates offer a number of advantages over colorimetric substrates.  With greater sensitivity than any other available detection method, chemiluminescence allows the ability to visualize protein bands at low detection limits.