HRP Chemiluminescent Substrates
BioFX Laboratories offers five unique HRP chemiluminescent substrates to meet all your sensitivity requirements for both microwell and membrane applications.  These two component formulations are characterized by varying lengths of light emission and intensity and achieve a 100-fold improvement in signal-to-noise ratio over existing luminol formulations.  Luminol is a versatile chemical that exhibits chemiluminescence (a striking blue glow) when mixed with an appropriate oxidizing agent. Light is produced only while the enzyme (HRP) and substrate reaction is occurring.  This is in contrast to colorimetric substrates, where a stable color is produced and the resulting precipitates remain on the membrane, even after the enzyme-substrate reaction has completed.  For Western blotting, chemiluminescent substrates offer a number of advantages over colorimetric substrates.  With greater sensitivity than any other available detection method, chemiluminescence allows the ability to visualize protein bands at low detection limits.